| Thedacare INC. | Neenah, WI | $167M | — | 2023 |
| Inclusa, INC. | Stevens Point, WI | $61M | 1,588 | 2024 |
| My Choice Wisconsin, INC. | Wauwatosa, WI | $51M | — | 2023 |
| Gustave A. Larson Company | Pewaukee, WI | $45M | — | 2023 |
| Davis & Kuelthau, S.c. | Milwaukee, WI | $36M | — | 2023 |
| Mid-states Aluminum Corp. | Fond Du Lac, WI | $31M | — | 2023 |
| Krohn Clinic, LTD. | Black River Falls, WI | $30M | 207 | 2025 |
| Curative Care Network, INC. | Milwaukee, WI | $28M | 408 | 2025 |
| Cbc Coating, INC. | Appleton, WI | $24M | — | 2023 |
| Credit Union National Association INC. | Madison, WI | $23M | — | 2023 |
| The F. Dohmen CO. | Milwaukee, WI | $23M | — | 2023 |
| Wisconsin Reinsurance Corporation | Madison, WI | $21M | 104 | 2024 |
| T.i.c., INC. | Onalaska, WI | $20M | 275 | 2025 |
| Inlanta Mortgage, INC | Waukesha, WI | $19M | — | 2023 |
| Brennan Steil S.c. | Janesville, WI | $16M | 35 | 2023 |
| Deltrol Corporation | Milwaukee, WI | $16M | — | 2023 |
| Revela Foods, LLC | Colby, WI | $15M | 384 | 2024 |
| Andis Company | Sturtevant, WI | $15M | — | 2024 |
| Wausau Heart & Lung Surgeons, S.c. | Wausau, WI | $14M | 4 | 2025 |
| Physicians Realty Trust | Milwaukee, WI | $14M | 125 | 2024 |
| Wilde Automotive Management Of Wisconsin, INC. | North Prairie, WI | $13M | — | 2023 |
| Salm Partners, LLC | Denmark, WI | $13M | 1,024 | 2024 |
| Wisconsin Institute Of Urology, Sc | Neenah, WI | $13M | 123 | 2025 |
| The Konop Companies, INC. | Green Bay, WI | $12M | 100 | 2024 |
| Worzella & Sons, INC. | Plover, WI | $12M | 27 | 2025 |
| Osi Environmental,llc | Waukesha, WI | $12M | 123 | 2025 |
| Kalmbach Media CO. | Waukesha, WI | $12M | 77 | 2025 |
| Radiology-waukesha, S.c. | Waukesha, WI | $12M | 31 | 2025 |
| Southwest Health Center, INC. | Platteville, WI | $12M | — | 2023 |
| Asenzya, INC. | Oak Creek, WI | $11M | 191 | 2024 |
| Zepnick Solutions, INC. | Green Bay, WI | $10M | 65 | 2024 |
| Westbury Bank | Waukesha, WI | $9.8M | — | 2023 |
| The Lake Companies, INC | Green Bay, WI | $9.7M | 61 | 2024 |
| Madison Emergency Physicians, S.c. | Madison, WI | $9.3M | 81 | 2023 |
| Financial Solutions LLC | Waukesha, WI | $9.2M | 13 | 2023 |
| National Rivet & Manufacturing CO. | Waupun, WI | $9.2M | 40 | 2024 |
| Salelytics, LLC | Appleton, WI | $9.2M | 277 | 2024 |
| Hb Performance Systems, INC. | Mequon, WI | $9.0M | 242 | 2025 |
| Common Ground Healthcare Cooperative | Brookfield, WI | $8.9M | 144 | 2025 |
| Sutton Transport, INC. | Wausau, WI | $8.7M | 622 | 2025 |
| Skyline Steel, INC. | Arlington, WI | $8.6M | 28 | 2024 |
| Process Retail Group, INC. | New Berlin, WI | $8.5M | 62 | 2024 |
| The Infosoft Group LLC | Milwaukee, WI | $8.4M | — | 2023 |
| Northeast Wisconsin Retina Associates, Sc | Appleton, WI | $8.1M | 54 | 2024 |
| Madison Oral And Maxillofacial Surgeons, Sc | Madison, WI | $8.0M | 45 | 2024 |
| Saris Cycling Group | Madison, WI | $7.9M | — | 2023 |
| Wisconsin Bone & Joint, S.c. | Wauwatosa, WI | $7.4M | 73 | 2024 |
| Service Specialists Of Dane County, INC. | Sun Prairie, WI | $7.3M | 77 | 2023 |
| Tailored Solutions INC. | Milwaukee, WI | $7.2M | 28 | 2024 |
| Radiology Chartered | Green Bay, WI | $7.1M | 16 | 2023 |
| Websters United Foods Services, INC. | Ripon, WI | $6.9M | 126 | 2025 |
| International Concrete Products, INC. | Germantown, WI | $6.9M | 66 | 2024 |
| First Choice Ingredients, INC. | Germantown, WI | $6.8M | 95 | 2023 |
| Bob Clapper Automotive, INC. | Janesville, WI | $6.8M | — | 2023 |
| W. W. Electric Motors, INC. | Oshkosh, WI | $6.6M | 33 | 2024 |
| Green Bay Insurance Center, INC. | Green Bay, WI | $6.6M | 24 | 2025 |
| Wisconsin Imaging, LLC | Little Chute, WI | $6.5M | 41 | 2024 |
| Body Shop Supply CO., INC. | Madison, WI | $6.5M | 42 | 2025 |
| Walkabout Orthotics & Prosthetics, INC. | Wausau, WI | $6.5M | 40 | 2024 |
| Unity Holdings, INC. | Fox Point, WI | $6.3M | 119 | 2025 |
| Parking Lot Maintenance, INC. | Pewaukee, WI | $6.3M | 113 | 2023 |
| Huebsch Laundry Company | Eau Claire, WI | $6.2M | 212 | 2025 |
| Carl F. Statz & Sons, INC. | Waunakee, WI | $6.2M | 39 | 2025 |
| Greater Milwaukee Plastic Surgeons, S.c. | Brookfield, WI | $6.1M | 8 | 2025 |
| Borg Indak, INC. | Delavan, WI | $6.1M | 165 | 2024 |
| Fleetworthy Solutions, INC. | Madison, WI | $6.0M | 166 | 2024 |
| Pritzlaff Wholesale Meats, INC. | New Berlin, WI | $6.0M | 45 | 2024 |
| Johnson Chiropractic Office, S.c. | Waupun, WI | $6.0M | 2 | 2024 |
| Current Technologies INC | Wisconsin Rapids, WI | $6.0M | 5 | 2025 |
| Madeline Island Ferry Line, INC. | Lapointe, WI | $5.9M | 37 | 2024 |
| Professional Mgmt Of Milwaukee INC | Brookfield, WI | $5.9M | 9 | 2024 |
| Antigo Construction, INC. | Antigo, WI | $5.8M | 26 | 2024 |
| Sks Machine | Wisconsin Rapids, WI | $5.8M | 30 | 2025 |
| Quality Air Forwarding, INC. | Milwaukee, WI | $5.7M | 18 | 2024 |
| Dimension X Design LLC | Eau Claire, WI | $5.7M | 18 | 2024 |
| Oral & Maxillofacial Surgery Fox Cities, S.c. | Appleton, WI | $5.7M | 45 | 2025 |
| Ecm Holding Group, INC. | Oshkosh, WI | $5.6M | 158 | 2025 |
| Wisconsin Plastic Products | Plymouth, WI | $5.6M | 122 | 2025 |
| Personnel Management Group, INC. | Hudson, WI | $5.5M | 70 | 2024 |
| Extract Systems, LLC | Madison, WI | $5.5M | 48 | 2024 |
| Timber Technologies, LLC | Colfax, WI | $5.4M | 41 | 2024 |
| Heyrman Construction Company, INC. | Green Bay, WI | $5.3M | 12 | 2025 |
| Center For Oral And Maxillofacial Surgery, S.c. | Madison, WI | $5.3M | 74 | 2025 |
| Artisan Dental, LLC | Madison, WI | $5.2M | 33 | 2024 |
| Pegasus Partners LTD. | Mequon, WI | $5.2M | 22 | 2025 |
| Blue Chip Retirement Plans, INC. | Milwaukee, WI | $5.2M | 17 | 2025 |
| Green & Gold Concrete, INC. | Green Bay, WI | $5.2M | 33 | 2024 |
| Douglas A. Krueger, D.D.S. | Appleton, WI | $5.1M | — | 2023 |
| Agility Holdings, LLC | Milwaukee, WI | $5.1M | 92 | 2024 |
| Stefan's Soccer Supply, INC. | Brookfield, WI | $5.1M | 72 | 2024 |
| Mandli Communications, INC. | Fitchburg, WI | $5.0M | 150 | 2024 |
| Automated Products, INC. | Marshfield, WI | $5.0M | 66 | 2024 |
| Dic Imaging Products USA LLC | Oak Creek, WI | $5.0M | 52 | 2023 |