| The Surgical Clinic, PLLC | Nashville, TN | $64M | 278 | 2025 |
| Dialysis Clinic, Inc. | Nashville, TN | $51M | 1,589 | 2024 |
| Neal & Harwell, PLC | Nashville, TN | $47M | 59 | 2025 |
| Hendrick Manufacturing Co. | Germantown, TN | $36M | 235 | 2024 |
| Pediatric Anesthesiologists, P.A. | Memphis, TN | $31M | 61 | 2023 |
| Lackie Trading, Inc. | Memphis, TN | $31M | 3 | 2024 |
| Novatech Inc. | Nashville, TN | $28M | 641 | 2024 |
| Pilot Corporation | Knoxville, TN | $18M | 2,769 | 2024 |
| Knoxville Gastrointestinal Specialists, P.C. | Knoxville, TN | $17M | 25 | 2025 |
| Old Harding Pediatric Associates, P.C. | Nashville, TN | $17M | 93 | 2025 |
| Phillip L. Nichols | Knoxville, TN | $15M | 18 | 2025 |
| Greater Knoxville Ent Associates, PC | Powell, TN | $14M | 90 | 2025 |
| Velocity Risk Underwriters, LLC | Nashville, TN | $14M | 205 | 2025 |
| Pathology Group of the Midsouth, P.C. | Germantown, TN | $14M | 47 | 2024 |
| Lpb Holding Company, Inc. | Memphis, TN | $13M | 81 | 2024 |
| Cripps, Hooper & Rhody, P.L.L.C. | Smithville, TN | $13M | 41 | 2025 |
| Bank of Cleveland | Cleveland, TN | $12M | 61 | 2025 |
| Tennessee Women's Care, P.C. | Franklin, TN | $11M | 79 | 2023 |
| Simplybank | Dayton, TN | $11M | 173 | 2024 |
| Qualderm Partners, LLC | Brentwood, TN | $11M | 65 | 2024 |
| Johnson City Urological Clinic, PC | Johnson City, TN | $9.5M | 52 | 2023 |
| Mountain Empire Eye Physicians, PLLC | Bristol, TN | $9.5M | 51 | 2023 |
| Associated Orthopaedics of Kingsport, P.C. | Kingsport, TN | $9.3M | 46 | 2025 |
| International Assurance of Tenne | Franklin, TN | $9.0M | 18 | 2023 |
| Frank E. Neal & Company, Inc. | Brentwood, TN | $8.7M | 22 | 2023 |
| Farris Bobango Branan PLC. | Memphis, TN | $8.5M | 32 | 2025 |
| Knoxville Pulmonary Group, LLC | Knoxville, TN | $8.3M | 11 | 2026 |
| Twin Islands Motel, Inc. | Gatlinburg, TN | $8.1M | 32 | 2023 |
| Gallaher & Associates, Inc. | Alcoa, TN | $7.8M | 119 | 2026 |
| Equinox Information Systems Inc | Madison, TN | $7.7M | 25 | 2024 |
| Pulmonary Associates of East Tennessee | Johnson City, TN | $7.5M | 26 | 2024 |
| Institutional Wholesale Co., Inc. | Cookeville, TN | $7.2M | 170 | 2024 |
| Banyas & Miranda D.D.S. | Johnson City, TN | $7.0M | 3 | 2023 |
| Trinisys, LLC | Brentwood, TN | $6.9M | 103 | 2023 |
| Cardiovascular Surgery Clinic PLLC | Memphis, TN | $6.8M | 43 | 2023 |
| Eti Corporation | Memphis, TN | $6.6M | 16 | 2026 |
| Associates In Oral & Maxillofacial Surgery, P.C. | Chattanooga, TN | $6.6M | 3 | 2023 |
| Acuff & Associates, Inc. | Brentwood, TN | $6.6M | 59 | 2024 |
| Brian S. Biesman, MD, PLLC | Nashville, TN | $6.5M | 15 | 2024 |
| Csc Investments, LLC | Cleveland, TN | $6.4M | 360 | 2023 |
| West Wilson Family Practice, P. C. | Mt. Juliet, TN | $6.3M | 30 | 2024 |
| Genetics Associates, Incorporated | Nashville, TN | $6.2M | 47 | 2024 |
| Chattanooga Family Practice Associates, P.C. | Chattanooga, TN | $6.2M | 37 | 2024 |
| J. W. Hudson, DDS | Knoxville, TN | $5.9M | 5 | 2025 |
| Trustcore, LLC | Brentwood, TN | $5.9M | 49 | 2023 |
| Equipment Finders, Inc. | Nashville, TN | $5.8M | 121 | 2026 |
| Klinke Bros. Ice Cream Co., Inc. | Memphis, TN | $5.7M | 13 | 2023 |
| Exedy America Corporation | Mascot, TN | $5.7M | 103 | 2025 |
| Eureka Foundry Company | Chattanooga, TN | $5.6M | 9 | 2024 |
| Custom Steel Fabricators, Inc. | Columbia, TN | $5.5M | 33 | 2024 |
| Walker Lumber & Hardware, Inc. | Nashville, TN | $5.5M | 76 | 2025 |
| L. Sean Mullins, D.D.S. | Bristol, TN | $5.5M | 14 | 2024 |
| Svrpc, Inc. | Knoxville, TN | $5.5M | 32 | 2023 |
| The Law Office of Cynthia L. Eldred A Professional Corporation | Maryville, TN | $5.4M | 2 | 2023 |
| Kaye Food Companies, LLC | Memphis, TN | $5.4M | 72 | 2025 |
| Scott & Pohlman, P.C. | Memphis, TN | $5.3M | 17 | 2025 |
| Rea, Inc. | Knoxville, TN | $5.3M | 67 | 2025 |
| Entech Engineering, Inc. | Brentwood, TN | $5.2M | 16 | 2025 |
| St. Paul Christian Academy, Inc. | Nashville, TN | $5.2M | 107 | 2024 |
| The Southern Co., Inc. | Memphis, TN | $5.2M | 71 | 2023 |
| B.H. Holmes Construction Company, Inc. | La Vergne, TN | $5.2M | 42 | 2024 |
| Taylor Financial Corporation | Brentwood, TN | $5.1M | 20 | 2025 |
| Frazee Ivy Davis, PLC | Memphis, TN | $5.1M | 35 | 2025 |
| Matt J. Gorham, Jr., D.D.S., P.C. | Nashville, TN | $5.1M | 18 | 2024 |
| Thomas P. Chu, M.D., P.C. | Cordova, TN | $5.0M | 3 | 2026 |