| Owensboro Grain Company, LLC | Owensboro, KY | $40M | 200 | 2023 |
| Middleton Reutlinger, P.S.C. | Louisville, KY | $35M | 124 | 2023 |
| Argi Holdings, Inc. | Louisville, KY | $20M | 308 | 2023 |
| Franklin Asset Management Co Inc | Louisville, KY | $20M | 17 | 2024 |
| Advanced Ent and Allergy, PLLC | Louisville, KY | $20M | 164 | 2024 |
| Virtus Precision Tube LLC | Franklin, KY | $18M | 148 | 2023 |
| Anesthesiology Associates, P.S.C. | Louisville, KY | $16M | 33 | 2023 |
| Dermatology Associates of Kentucky, P.S.C. | Lexington, KY | $16M | 97 | 2023 |
| Champion Petfoods USA Inc. | Auburn, KY | $15M | 381 | 2023 |
| Probank Austin | Prospect, KY | $14M | 74 | 2023 |
| Kleinert, Kutz & Associates Hand Care Ctr, PLLC | Louisville, KY | $14M | 163 | 2024 |
| Pediatric & Adolescent Associates P.S.C. | Lexington, KY | $12M | 66 | 2024 |
| G & S Services, Inc. | Covington, KY | $12M | 3 | 2025 |
| Rah Ventures, Inc. | Louisville, KY | $12M | 1 | 2024 |
| Anesthesia & Pain Specialists of Bowling Green, PLC | Bowling Green, KY | $11M | 122 | 2024 |
| Lexington Surgical Specialists, P.S.C. | Lexington, KY | $11M | 24 | 2026 |
| The Lang Company | Louisville, KY | $10M | 65 | 2024 |
| Kv Oil & Gas, LLC | Lexington, KY | $10M | 3 | 2025 |
| George C Borst, III, Psc | Ashland, KY | $10M | 35 | 2024 |
| First State Bank of the Southeast, Inc. | Middlesboro, KY | $9.7M | 93 | 2025 |
| Rkneal, LLC | Paducah, KY | $9.7M | 138 | 2025 |
| Power Graphics, Inc. | Louisville, KY | $9.6M | 85 | 2023 |
| Emergency Medical Associates, P.S.C. | Louisville, KY | $9.4M | 24 | 2023 |
| Surgical Care Associates Psc | Louisville, KY | $9.2M | 21 | 2026 |
| Purchase Anesthesia, Psc | Paducah, KY | $8.9M | 28 | 2024 |
| Russellville Engineered Castings, Inc. | Russellville, KY | $8.1M | 198 | 2026 |
| Marsh Building Products, Inc. | Fort Thomas, KY | $7.8M | 106 | 2023 |
| Pikeville Radiology, PLLC | Pikeville, KY | $7.8M | 16 | 2024 |
| Anesthesiology Associates, P.S.C. | Louisville, KY | $7.7M | 32 | 2023 |
| Bluegrass Newborn Specialists, PLLC | Lexington, KY | $7.7M | 7 | 2024 |
| Kenneth G. Cook, M.D., Psc | Paducah, KY | $7.4M | 4 | 2024 |
| Premier Medical Group | Owensboro, KY | $7.3M | 54 | 2026 |
| Erdmann Corporation | Louisville, KY | $7.2M | 32 | 2024 |
| Paul Michels & Sons, Inc. Aka Michels Construction | Ft. Mitchell, KY | $7.0M | 77 | 2023 |
| Vinland Energy Operations, LLC | Lexington, KY | $6.9M | 111 | 2024 |
| Kentucky Medical Services Foundation, Inc. | Lexington, KY | $6.7M | 335 | 2024 |
| Owensboro Dermatology Associates, P.S.C. | Owensboro, KY | $6.7M | 89 | 2024 |
| Heartland Health System, Inc. | Louisville, KY | $6.4M | 356 | 2023 |
| Brown Wood Preserving Company, Inc. | Prospect, KY | $6.3M | 72 | 2024 |
| Curtis Rafferty | Bowling Green, KY | $6.2M | 3 | 2025 |
| Metro Louisville Endodontics, LLC | Louisville, KY | $6.2M | 6 | 2026 |
| Bates Security, LLC | Lexington, KY | $6.1M | 118 | 2024 |
| Insuramax, Inc. | Louisville, KY | $6.0M | 54 | 2024 |
| Robert Haragan, Inc. | Louisville, KY | $6.0M | 45 | 2024 |
| Hemmer Wessels McMurtry, PLLC | Fort Mitchell, KY | $6.0M | 16 | 2026 |
| Bluegrass Retina Consultants, Psc | Lexington, KY | $5.8M | 20 | 2025 |
| Bluegrass Orthopaedics, Psc | Lexington, KY | $5.8M | 211 | 2024 |
| The Womens Pavilion, P.S.C. | Owensboro, KY | $5.7M | 18 | 2023 |
| Access Physical Therapy & Wellne | Louisville, KY | $5.5M | 418 | 2024 |
| Harper Industries Inc. | Paducah, KY | $5.5M | 80 | 2025 |
| Maysville Radiology, Psc | Lexington, KY | $5.5M | 3 | 2024 |
| Kingsport Urology Group, PC | Lexington, KY | $5.2M | 13 | 2024 |
| Networld Alliance, LLC | Louisville, KY | $5.2M | 36 | 2026 |
| Bluegrass Kesco, Inc. | Louisville, KY | $5.1M | 34 | 2024 |
| Barnett Benvenuti & Butler, PLLC | Lexington, KY | $5.1M | 9 | 2024 |