| Parkview Medical Center, Inc. | Pueblo, CO | $297M | 3,626 | 2024 |
| Clovis Oncology, Inc. | Boulder, CO | $52M | 365 | 2023 |
| Heska Corporation | Loveland, CO | $47M | 568 | 2023 |
| Cherry Creek Mortgage, LLC | Greenwood Village, CO | $43M | 933 | 2023 |
| Pattlen Enterprises Inc. | Denver, CO | $33M | 121 | 2025 |
| Revenir Energy Services LLC | Denver, CO | $26M | 195 | 2024 |
| Honnen Equipment Company | Commerce City, CO | $25M | 335 | 2024 |
| Group Publishing Inc. | Loveland, CO | $22M | 156 | 2024 |
| Albert Frei & Sons, Inc. | Evergreen, CO | $22M | 103 | 2024 |
| Mpeg La, LLC | Greenwood Village, CO | $21M | 75 | 2023 |
| Critigen LLC | Denver, CO | $21M | 281 | 2024 |
| Universal Lending Corporation | Denver, CO | $19M | 154 | 2024 |
| Thirdera, LLC | Broomfield, CO | $17M | 255 | 2024 |
| Colorado Ob/Gyn Partners, PLLC | Denver, CO | $16M | 207 | 2023 |
| Carepoint Health, LLC | Greenwood Village, CO | $16M | 176 | 2024 |
| Peak Resources | Denver, CO | $15M | 50 | 2024 |
| Southern Colorado Heart & Vascular, PC DBA Colorado Springs Cardiologi | Colorado Springs, CO | $14M | 55 | 2024 |
| Valdez International Corporation | Colorado Springs, CO | $14M | 306 | 2024 |
| Aimc, P.C. | Centennial, CO | $13M | 14 | 2026 |
| Pb Tech & Advisory LLC | Denver, CO | $13M | 274 | 2024 |
| Cdw Corporation | Parker, CO | $12M | 3 | 2024 |
| Pioneer Sand Company, Inc. | Highlands Ranch, CO | $11M | 598 | 2024 |
| Treece Alfrey Musat P.C. | Denver, CO | $11M | 31 | 2025 |
| Bank of Denver | Denver, CO | $10M | 40 | 2024 |
| Dundee Resort Dev LLC DBA Arapahoe Basin Ski | Dillon, CO | $10M | 259 | 2025 |
| Tracegains Inc | Broomfield, CO | $9.7M | 156 | 2025 |
| Canoe Ventures LLC | Lakewood, CO | $9.7M | 46 | 2024 |
| Biggskofford PC | Colorado Springs, CO | $9.5M | 57 | 2026 |
| Codebex, Inc. | Evergreen, CO | $9.2M | 10 | 2025 |
| Qsi Consulting, Inc. | Denver, CO | $9.2M | 11 | 2025 |
| Metcalf Archaeological Consultants, Inc. | Golden, CO | $8.8M | 57 | 2025 |
| Bscs Science Learning | Colorado Springs, CO | $8.7M | 129 | 2023 |
| Cybergrx, Inc. | Denver, CO | $8.2M | 226 | 2023 |
| Lightning Emotors, Inc | Loveland, CO | $8.1M | 357 | 2024 |
| Taft Stettinius & Hollister LLP | Denver, CO | $7.7M | 38 | 2025 |
| Taft Stettinius & Hollister LLP | Denver, CO | $7.7M | 38 | 2025 |
| Redstone Strategy Group, LLC | Boulder, CO | $7.7M | 54 | 2023 |
| James M Johnston & Associates, Inc. | Glendale, CO | $7.4M | 3 | 2024 |
| Terrence P. Murphy, M.D., P.C. | Englewood, CO | $7.3M | 18 | 2025 |
| M.A. Concrete Construction, Inc. | Grand Junction, CO | $7.2M | 56 | 2025 |
| Anderson Consulting Engineers Inc. | Fort Collins, CO | $6.9M | 17 | 2025 |
| Erickson, Brown & Kloster, LLC | Colorado Springs, CO | $6.7M | 84 | 2024 |
| Freight All Kinds, Inc. | Denver, CO | $6.6M | 62 | 2023 |
| Law Office of Neil Otoole P. C. | Denver, CO | $6.6M | 8 | 2024 |
| Ponderosa Associates, Ltd. | Lafayette, CO | $6.4M | 16 | 2023 |
| The Sisters of St. Francis, Colorado Springs, Inc | Colorado Springs, CO | $6.3M | 181 | 2023 |
| Colorado Physician Partners, PLLC | Denver, CO | $6.3M | 143 | 2023 |
| James F Reppert | Canon City, CO | $6.2M | 2 | 2023 |
| Quality Lapel Pins, Inc. | Littleton, CO | $6.2M | 6 | 2023 |
| Vail-Summit Orthopaedics, PC | Frisco, CO | $6.1M | 134 | 2023 |
| Educational Measures, LLC | Greenwood Village, CO | $6.0M | 112 | 2025 |
| Ecological Resource Consultants, Inc. | Lakewood, CO | $5.9M | 17 | 2024 |
| Nalin J. Mehta MD, PC | Golden, CO | $5.9M | 3 | 2024 |
| Spradley-Barr Ford, Inc. | Fort Collins, CO | $5.9M | 75 | 2023 |
| Yampa Valley Medical Associates | Steamboat Springs, CO | $5.9M | 36 | 2025 |
| National Business Media Inc. | Westminster, CO | $5.8M | 56 | 2023 |
| Maccourt Products, Inc. | Endglewood, CO | $5.7M | 31 | 2026 |
| Quality Lapel Pins, Inc. | Littleton, CO | $5.7M | 6 | 2024 |
| Metro Appliance Service, Inc. | Denver, CO | $5.6M | 6 | 2026 |
| Digital Retirement Solutions | Longmont, CO | $5.6M | 24 | 2023 |
| Iconergy Ltd. | Denver, CO | $5.6M | 68 | 2025 |
| The Colorado Floor Company, Inc. | Arvada, CO | $5.5M | 94 | 2025 |
| Atmospheric and Space Technology Research Associates, LLC | Louisville, CO | $5.5M | 112 | 2024 |
| Tengam Pro, Inc. | Castle Rock, CO | $5.5M | 122 | 2025 |
| Wells Anderson & Race, LLC | Denver, CO | $5.4M | 33 | 2025 |
| High Valley Group, Inc. | Colorado Springs, CO | $5.4M | 5 | 2023 |
| Graham Oleson | Colorado Springs, CO | $5.3M | 67 | 2024 |
| Kerr & Associates, Inc. | Monument, CO | $5.3M | 6 | 2023 |
| Special Aerospace Services | Boulder, CO | $5.1M | 152 | 2025 |
| Brock USA, LLC | Boulder, CO | $5.1M | 33 | 2025 |
| Lifescape Associates, Inc. | Denver, CO | $5.0M | 74 | 2024 |