| Birmingham-Southern College | Birmingham, AL | $91M | 893 | 2025 |
| Decatur Orthopaedic Clinic | Decatur, AL | $17M | 72 | 2025 |
| Montgomery Rheumatology Assoc.,p.a. | Montgomery, AL | $16M | 8 | 2025 |
| Cullman Primary Care, P.C. | Cullman, AL | $15M | 226 | 2024 |
| Wrm Group, LLC | Jasper, AL | $15M | 97 | 2024 |
| Tidwell Group, LLC | Birmingham, AL | $14M | 199 | 2024 |
| Alabama Womens Specialists PC | Birmingham, AL | $13M | 31 | 2024 |
| Huntsville Emergency Medical Services, Inc. | Huntsville, AL | $12M | 345 | 2024 |
| Pose Group, Inc. | Daphne, AL | $12M | 623 | 2024 |
| Michael D. Edwards, D.M.D., P.C. | Wedowee, AL | $11M | 6 | 2025 |
| Baldwin Pole & Piling Company, Inc. | Bay Minette, AL | $11M | 59 | 2023 |
| Shah Clinic of Ne Alabama, L.L.C. | Gadsden, AL | $11M | 5 | 2024 |
| McKinney Equipment, Inc. | Mobile, AL | $10M | 32 | 2023 |
| Pulmonary & Sleep Associates of Huntsville, P.C. | Huntsville, AL | $9.0M | 43 | 2025 |
| Fountain, Parker, Harbarger & Associates, LLC | Huntsville, AL | $8.3M | 34 | 2024 |
| Winston Home Builders, LLC | Double Springs, AL | $7.9M | 117 | 2024 |
| Mrb Law, LLC | Birmingham, AL | $7.5M | 40 | 2025 |
| Russell H Lowrey DMD PC | Huntsville, AL | $7.5M | 2 | 2024 |
| Manderson Construction Company, Inc | Tuscaloosa, AL | $7.0M | 3 | 2024 |
| James C. Grotting, M.D., P.C. | Birmingham, AL | $6.8M | 7 | 2025 |
| National Copper, LLC | Huntsville, AL | $6.7M | 162 | 2023 |
| Burkholder Insurance, Inc. | Mobile, AL | $6.5M | 16 | 2023 |
| Mdg Professional Group, PC | Huntsville, AL | $6.5M | 33 | 2023 |
| Hawkins, Hawkins, Gales, and Harris Dentistry Partnership | Decatur, AL | $6.4M | 75 | 2023 |
| Anesthesia Medical Consultants, LLC | Sheffield, AL | $6.1M | 11 | 2023 |
| P & R, L.L.C. | Stapleton, AL | $5.9M | 42 | 2025 |
| Ob/Gyn Associates of Alabama, PC | Birmingham, AL | $5.9M | 52 | 2025 |
| University Surgical Associates PC | Tuscaloosa, AL | $5.9M | 20 | 2024 |
| Family Health Associates, P.C. | Jasper, AL | $5.8M | 39 | 2023 |
| Gastroenterologists, P.C. | Fairhiope, AL | $5.8M | 19 | 2024 |
| Fuqua & Partners Architects, PC | Huntsville, AL | $5.8M | 32 | 2025 |
| Ronnie Salloway & Company, Inc. | Sylacauga, AL | $5.7M | 6 | 2024 |
| Sequal Youth and Family Services | Huntsville, AL | $5.7M | 1,458 | 2023 |
| Lauderdale Radiology Group, LLP | Florence, AL | $5.5M | 14 | 2025 |
| Valley Machine Company, Inc. | Alexandria, AL | $5.4M | 75 | 2025 |
| Axim Geospatial, LLC | Birmingham, AL | $5.4M | 234 | 2023 |
| Michael L. Putman, M.D., P.A. | Decatur, AL | $5.4M | 5 | 2024 |
| Kmkg Anesthesia, P.C. | Jasper, AL | $5.3M | 26 | 2023 |
| Flowers Insurance Agency, LLC | Dothan, AL | $5.2M | 37 | 2024 |
| Retina Consultants of Al, P.C. | Birmingham, AL | $5.2M | 112 | 2025 |
| Maxillofacial Surgery Center of Huntsville, Inc. | Huntsville, AL | $5.1M | 6 | 2025 |
| Mobile Gastroenterology, P.C. | Mobile, AL | $5.0M | 3 | 2025 |
| Southern Community Bancshares, Inc. | Cullman, AL | $5.0M | 14 | 2025 |
| Brady Martin, L.L.P. | Mobile, AL | $5.0M | 5 | 2024 |
| Andrews Sports Medicine and Orthopaedic Center, LLC | Birmingham, AL | $5.0M | 130 | 2024 |